Diarrhea does not necessarily indicate Nosema. There can be other factors involved. Are you noticing any other problems with your hive that may point towards an infection? The best way is to examine with a microscope. The USDA's Bee Research Lab in Beltsville, MD provides a free service. However due to the virus plaguing our nation they may be backlogged.I've never had to deal with nosema and was wondering if yellow diarrhea indicates nosema? I'd heard brown diarrhea did indicate nosema but not yellow. Is that correct?
Your in Latrobe and making a split already. Even with a mated queen that seems early for this area. If this works out for you I will need to re-evaluate my timing of things. Wish you the best.I fed pollen sub around Jan or Feb. This is a very strong hive and I was in the process of making a split. I added a 3rd hive box above the 2 already there. I'm wondering if there was anything in the drawn frames I added. Only thing I can think of is there was a small patch of dead bees (heads in cells) from 2 years ago on one frame.