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Lee’s Summit, MO.
I do the same as Ravenseye but I'm under the impression the reapplication is not necessary as the bees don't/can't remove the spores. However, if in doubt as to if a respective frame has or has not been treated I will reapply.

Nothing like seeing a tiny start of wax moth larva webbing that simply peters out. I smile knowing it ate one of those spores and the rest is history.
 
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