I don't recall the mix but I think it was more like tablespoons than teaspoons. When I use it, I mix it in a hand held sprayer and spray all the combs before I stack them in the barn. I don't reapply annually unless the combs have gone back out to the hive and then in again.
I do the same as Ravenseye but I'm under the impression the reapplication is not necessary as the bees don't/can't remove the spores. However, if in doubt as to if a respective frame has or has not been treated I will reapply.
Nothing like seeing a tiny start of wax moth larva webbing that simply peters out. I smile knowing it ate one of those spores and the rest is history.
On a previous thread it was stated that four teaspoons per gallon was the max amount. I use only two and have had very good results. In the fall when I find several frames of empty comb in a hive I take them out, spray them and put them back wet. I have seen no ill effects and have eliminated any major wax moth problems.
2 teaspoons per gallon of water, 3 teaspoons is a strong mix.
I mix a gallon at a time and use a hand sprayer. One quart will cover both sides of about 30 deep frames. Just need to get a good mist into the bottom of the cells, let it air dry before stacking up to prevent mold. Mix only what you will use in a three day period, otherwise it will go rancid. The spores will last until you put the frames back into production, then the bees will polish out the cells. In the dry powder form the shelf life is many years, keep cool and dry.
I had about 5 shallow supers that got sprayed with certan from Kelley's (that should tell you how long ago that was) stacked up and forgotten in my Mom's garage for close to 20 years, looked as good as the day it was put away.
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