sovek,
three weeks from emergence is about what most folks use as a benchmark, but you can get a late bloomer sometimes.
worse than being queenless would be if there is a defective queen in the hive, (poorly raised or poorly mated or both).
i have had two instances in which i thought the hive was queenless, only to have the bees reject and kill an introduced queen.
in one case, the hive had a queen and knew it, but that queen was a runt not much bigger than a worker. after they killed my introduced queen, i shook the colony out and made them re-enter the hive through an excluder. i found the runt on the bottom board and pinched her, and they accepted the next queen no problem.
the other case involved the bees appearing to have failed to make a new queen after swarming late in the season. the new queen happened to come in on about our summer dearth, when brood-rearing takes a break. again they killed my introduced queen because they had one, and the one they had started laying a few weeks later and turned out to be awesome.
the best test to see if your split is queenright is to give a frame of brood with just hatched larvae and/or eggs and see what they do with it. if they are queenless or have a poor queen they will likely start making queen cells on it and ultimately end up with a queen. you also buy some time to see if they have a laying queen without risking laying workers.
three weeks from emergence is about what most folks use as a benchmark, but you can get a late bloomer sometimes.
worse than being queenless would be if there is a defective queen in the hive, (poorly raised or poorly mated or both).
i have had two instances in which i thought the hive was queenless, only to have the bees reject and kill an introduced queen.
in one case, the hive had a queen and knew it, but that queen was a runt not much bigger than a worker. after they killed my introduced queen, i shook the colony out and made them re-enter the hive through an excluder. i found the runt on the bottom board and pinched her, and they accepted the next queen no problem.
the other case involved the bees appearing to have failed to make a new queen after swarming late in the season. the new queen happened to come in on about our summer dearth, when brood-rearing takes a break. again they killed my introduced queen because they had one, and the one they had started laying a few weeks later and turned out to be awesome.
the best test to see if your split is queenright is to give a frame of brood with just hatched larvae and/or eggs and see what they do with it. if they are queenless or have a poor queen they will likely start making queen cells on it and ultimately end up with a queen. you also buy some time to see if they have a laying queen without risking laying workers.