I would leave the virgin queens in there. I would expect them to lay in a week or less once they hatch. They will lay like crazy to. You won't be to far behind. I moved a large hive of mine and something happened to the new queen. They made several new queen and they were hatching right in front of my eyes. When one queen found the other it was last one standing!! I would be careful of putting another queen up in there. They will probably roll her and kill her. Check about every two weeks for queen cells. Usuall check the bottom of the frame of the top box that doesn't have a queen excluder. I pull out 3 frames from the middle and look. Most likely if you don't see queen cells than there isn't any. This keeps you from searching every frame. There could be more but with 10 hive and checking every frame you will get tired quick! Good luck and it is only a small set back. Plenty of time let in the flow to catch up!!