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If I want to test for presence/absence of queen by placing a frame of eggs in a hive, will the bees make emergency cells with the new eggs if a virgin queen is in the hive? I don't want the bees to make new queen but just to test for the presence of a queen, and to prevent laying workers IF the hive is indeed queenless. If it is queenless I'd remove the queen cell and place a mated queen, so as not to lose another month of brood production. What happens when there is a virgin queen in the hive? (Since supposedly virgin queens do not produce queen pheromone as do mated queens.)
 

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If there is a virgin queen they will not make emergency cells.
 

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(Since supposedly virgin queens do not produce queen pheromone as do mated queens.)
Virgin queens do produce a small amount of Queen Mandibular Pheromone. This QMP helps drones find virgin queens in the drone congregation areas. However, a virgin does not produce as much QMP as a mated queen.
 
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