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This will be my first nuc that I have done I have a mated queen from strachen apiaries in a 3 hole cage my question is can I introduce the queen immediatly or do you have to wait and release her in a day or so like normal?
 

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You need to allow the bees to release her as you normally would. Some Beeks do direct release if the hive has been queenless for a day or two , but most allow them to release in normal way. If the queen was a virgin queen, under most cases direct release can be done without any problems.
 

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I am assuming you are doing the split from one of your hives. In which case the bees may see the new queen as an invader, leave them queenless for a few hours and then leave the queen in the cage with the candy plug do what you would do with a package.
 
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