If you are referring to frames of a "normal" design that are 1 3/8" or 1 1/4" between the frame shoulders (widest parts of the end bars), the end bars should be pushed tight together to get the combs drawn out. Once you have drawn combs, for honey supers you may wish to space them out further. Frames in the brood nest are normally always touching the next frame and centered in the box.
If you mean the gap between the top bars, anything between 1/4" and 3/8" works fine. If you mean center to center spacing, I like 1 1/4". Anything over 1 3/8" is problematic in the brood nest. Anything under 1 1/8" is problematic.
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