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In theory, yes, but thats usually not recommended. When making queens from your eggs, you want to set up something called a cell-builder, which is a queenless hive with TONS of young worker bees that can produce as much royal jelly as possible. The queen larvae is fed something like over 1000 times per day and that requires a lot of bee-power. Once the queen cells have been capped however, then you can put them in any size nuc that you want.