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Bees can pass through a 1/4" space without a problem. Some people use hive entrance mouse guards of 1/4" wire mesh which actually has smaller 'holes' than 1/4" (minus half the thickness of each wire.)
Note that 1 1/4" frames and foundationless frames are really separate issues. You certainly can use them together, but there is no reason you have to.
Note that 1 1/4" frames and foundationless frames are really separate issues. You certainly can use them together, but there is no reason you have to.