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Queens Crapping Out

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I am interested in some opinions - I made 35 NUCs last month with new caged queens from a reputable source. Queens with few exceptions were well accepted and began laying nicely. After 1 brood cycle, I noticed that a rather significant number of these queens must have been killed and the NUCs begain to make supercedure cells to replace these queens. Thoughts?
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You say the queens "must have been killed" are you basing this on seeing cells started? Are there eggs still being laid? I am just getting at the difference between emergency and supercedure. In supercedure the bees do not kill the queen at least till her replacement is laying.
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