I attempted three queens using three nucs. Two failed apparently at stage queens took mating flights. Behavior of failed nucs markedly different from queenright neighbor. Queenless nucs more agitated as I worked them with lots of wing flapping while in comb. Workers also had abdomens up as is releasing pheromone. Behavior in some ways almost as if queenless colonies have a fever. They still bring in a little pollen but much less than queen right nuc.