I do all the time with no problems. That is as long as the queen isn't on the frame, then i put her off first. The brood hatches, develops and emerges just fine. IMO it's easier than dealing with the brushing of bees. Seems like it just makes them mad at me when I try that. But shaking does the trick every time... If you are looking for eggs, blow on the bees and they will move till you can see below..Can eggs in cells withstand a few very gentle shakes of the frame? Are eggs anchored to the bottom of the cell in some way? Thanks.