I found and marked the queen in my split while I was pulling the frames. I feel quite proud. She was a little miffed but just went right back down between the frames when I put her down. If only I could find the one in the mother hive, but man alive, that thing is FULL of bees! I'm guessing she spends most of her time in the upper deep, though, since the bottom seems to be full of nothing but honey and pollen. One of these days..