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I, two years ago, had 2 single deep 9 frame test hives where the (marked queens) were held behind excluders on 3 frames. I’ve marked frames of brood and watched the development from egg to emerging bee.
I vaporized those 2 hives EVERY time I’m went to the yard which was almost every week from March through October. I could find no harm to either the queens or brood during that time.

That was all I needed to see as to whether OAV was detrimental.
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