If the colony is queenless they will start to select larvae on the frame of brood given them as soon as the brood is placed in the colony. They start feeding the larvae and will begin to extend the cell walls to make the queen cell. Usually this process is far enough along to be seen in 24 hours or less, but with some colonies with strong adult populations the beekeeper misses the cell because it is covered by the bees. I would check in 48 hours when the cells are large enough to easily be seen.
Thanks for the reply, I'm was trying to decide between this or just going and buying another queen. I'll give them another week or so. I'll probably learn more watching them raise a queen than I would just buying one.
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