I just got a cage with 10 new queens I'm going to use for my splits and new packages arriving in a couple days. So the cage is sitting on my desk and these queens are piping like crazy from top of heir lungs! One is especially vocal, 2-3 sort of follow, the rest is just a choir. I read somewhere they are doing it as a "var" call on other queens. Is that what it is? They smell each other and call to fight? I wonder if aggressiveness of their calls somehow related to there ability level? But, any way, it's interesting to observe it.