It really makes no difference when you move it as long as you do so when all the bees are home. some like to move them at dark, and some in the morning. For a combine I generally close them up in the wee hours of the morning before daylight brings on activity. Then when I get to the new location I can do my combine in daylight. By doing it this way I only make one trip to either Apiary. Moving them in the evening, requires you to return the next day to do the combine, or do it in the dark, which makes for some angry bees.