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I lost one as well. It was a swarm I caught back in May. They went queenless in the beginning I got them a new queen laying good. Pulled some brood from them that I wanted to use for some queen rearing. I then decided to give them all frames of drawn comb so they would have a better chance of building up for winter. Poof two weeks later gone! About a nuc full of bees. They left brood it's still emerging. I blame myself. Nosy beekeeper. :no: