I'm not sure if I follow exactly what you are doing. It sounds as though you have a queen that has been accepted in a four frame nuc. Is that correct? Now you want to combine that four frame nuc with a larger hive? I would probably go back through the single deep and check for queen cells, and destroy any that you find. Then you could probably use the newspaper method to combine the two hives. 24 hours is usually plenty of time for them to know they are queenless. Unfortunately it is also enough time to start queen cells, which could hinder the acceptance of your new queen.