I made a 5 frame Nuc a month ago with a new marked queen I purchased. After 3 days they still didn't eat through the candy so I removed the cork to release her. A week later I tried to find her but could not. So I waited a few more day and still no signs of a queen. No eggs no larva. I never lost a queen this way so I introduced a frame with some fresh eggs to see if they would start queen cells which would tell me that they were queen-less. The eggs turned into larva and no queen cells, which tells me they have a queen? There are no eggs or larva on the frames when I first made the Nuc. So is there a queen or not? I should have been able to find her. She is marked and its Nuc. only 5 frames. Do I try to introduce another queen?