I am planing to make a couple hundred Nucs up this spring. I would like to make the Nucs up over several days then introduce virgin queens to the Nucs on the same day. My question is how long can a three frame nuc sit queenless before introducing a virgin queen? I want to incubate the cells and use only the best virgins. I have made this many Nucs before by placing queen cells but I think I will have a better success rate by eliminating the poor quality virgins before they are installed into the Nucs.