I made a queenless nuc over a month ago. I have grafted larva several times and by the 2nd day the bees have removed it. I have used new queen cups 3 times. I have left the cups in the nuc for a few days before grafting. I only take about 10 minutes to graft before putting the frame in the nuc. I have not used royal jelly to float them in but from my research I haven't seen that that is important. If anyone can help me please do.