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Disinfection of wooden structures contaminated
with Paenibacillus larvae subsp. larvae spores
W. Dobbelaere1,2, D.C. de Graaf1, W. Reybroeck3, E. Desmedt2, J.E. Peeters1
and F.J. Jacobs2
1Veterinary and Agrochemical Research Centre, Brussels, 2Laboratory for Zoophysiology, University of Ghent,
Ghent, 3Department Animal Product Quality, Agricultural Research Centre ± Ghent, Melle, Belgium
723/01/01: received 12 January 2001, revised 2 March 2001 and accepted 2 March 2001
W. DOBBELAERE, D.C. DE GRAAF, W. REYBROECK, E. DESMEDT, J.E. PEETERS
AND F. J . JACOBS. 2001.
Aims: The aim of the study is to examine the disinfection of wood contaminated with
Paenibacillus larvae subsp. larvae spores, in order to ®nd a practical decontamination method
for hive materials.
Methods and Results: The number of viable spores recovered after the treatment, on the
surface by swabbing, and in the deeper parts of the wood by scraping, was used to test the
ef®ciency of the disinfection. Our results indicate that chemical disinfection is only complete
when high concentrations (> 50%) of the disinfectant are used. Heat treatment in general was
found to be very effective. The scorching of wood was not satisfactory as it only killed spores at
the surface.
Conclusions: Complete disinfection is only possible with some heat treatments or by using
high concentrations of chemical disinfectants.
Signi®cance and Impact of the Study: This study puts forward some methods that can
provide complete decontamination, which is necessary for an effective control of American
foulbrood disease.