The beekeepers here in England...I'm visiting...are very up on biology. This for taking their "modules", on the way to taking the national exam. I was told today, that the egg shell is open a bit on one end so the sperm can enter. It's this end that is attached to the cell bottom when the queen lays the egg. Difficult to understand how an egg already in a cell would be fertilized under such circumstances...whether or not you added diluted honey to the cell to, in some way, soften the egg shell to make it permeable.
So you are saying that the open bit is effectively sealed off by the process of being attached to the bottom of the cell?
Maybe the diluted honey does not actually soften the "shell", but maybe it is helpful in "re-exposing" a tiny part of the open bit of the egg and allowing some access to fertilize the egg? If the honey is able to dissolve some of the adhesive material the queen uses to attach the egg--even by just a tiny bit--it seems conceivable that this process might work if you had freshly laid eggs to work with? Of course, what I have just written is speculation on what might be happening in cases where this process ends up working.
I think that this is worth investigating and could be an inexpensive way to do some line breeding.
To be honest--if it were someone other than Michael Bush who posted that this process can work, I think I would be just as skeptical as the harshest criticism posted on this thread.
I do think that the process would be difficult (maybe not as difficult as II, but still difficult). Now what would really be a feat would be to raise a few queens with this method described in this thread and then use II to further control the drones used. In this case, if you were able to make sure you had only genetic material from one queen (i.e. if all drones and drone eggs used are from the same queen), do you suppose that any resulting viable worker offspring would make a few tiny banjos and start playing "Dueling Banjos" out on the landing board? Or does it take one more round before they pull out the banjos? :lpf: