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Last week captured the bees from a downed tree. Have not seen a queen and doubt that she is in the hive....bees are bringing in pollen and making some comb. In taking open brood and eggs from another colony should I shake off all the nurse bees or leave them on?
 

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Yes, leave the nurse bees on, they won't pick any fights. Its mostly the guard bees and foragers that will get into little bee fistfights, so try to take the frame during the middle of the day when the foragers are out foraging and you should be fine. Just make double extra sure you don't have your own queen on that frame when you do it.
 
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