I have finally go through testing all of my colonies and found this one near the end. ~60 mites in a 300 bee alcohol wash and DWV. Here are a couple of pics. I treated with MAQS and will also requeen assuming the colony looks like it can recover post treatment. This is a good example of quickly things can go badly. This colony filled and capped two full supers and third super of comb honey in July. Two weeks later its crashing badly.