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usually your first drone brood will not have near the mite count after your first drone comb is sealed place you a second built drone comb in place when it is sealed pull it out usually this second drone comb will have lot more mites in it but before you leave it out i would do a mite check in the brood randomly uncap and pull the pupa out and look for mites 1 cell out of 10 cells with a mite you have a 10% infestation so uncap about 30 -40 cell to see what % infestation you have don't want over 15% or 20%. NOT OVER 15% is better.