I have done it quite a few times just by pulling the division board out. If you have a queen in both hives you might want to give a thought about which one you want to keep or just roll the dice knowing the younger one will take the crown about 75% of the time. There is some chance both queens could be injured but I think that is remote.
If I want to select one queen I will shake the other box through a queen excluder.
I have never had problems with fighting but I have never had determined robber bees. If you have a dearth going on and active robbing it would be an idea to use a layer or two of newspaper.
Supposedly or I should say commonly the queens and bees will be mother daughter or sisters but I sometimes have chosen brood from another hive to create the queen. The bees have never made an issue about it.
I had a bad experience with just removing the screen board, had quite a few dead bees on the bottom board the next day. I do a paper combine after the screen board if the bees came off a queened hive, just the newspaper if they’ve been queenless.
The only time I've used a double-screen board is when I've wanted to keep two queens from coming into contact with each other. When engaging in combining two colonies I use a single screen board, and then remove it after a few days to let all the bees mix.
It appears that the outcome will depend on the relationship of the colonies, how long they have shared the double screen division board, whether they are both queenright etc. We answered a question without enough supplied information.
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