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I've heard some beekeepers say it does but i haven't had any problems with it. If you are using it with sugar water to spray your bees with you can mix it to strong and they will beard on the outside of the hive and become more defensive. Jack
 

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I fed thyme oil to 5 packages this spring... two of the hives were extremely gentle and I worked them [still do] in shirt sleeves and without a hood. Two others were [and still are] pretty aggressive. The 5th was very easy to work, worked it today w/o hood... I'm still using thyme oil in this hive because it came with pretty serious dysentery. No dysentery now, might be the thyme oil or just recovering from the stress of being packaged and stuck in the hive for 2 days with cold/rain. So my conclusion is that thyme oil has no effect on aggressiveness.
 
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