Barring the abnormal situation in the colony, the queen would be shutting down anyway. With too few bees to do a mite wash it I would say the situation is beyond grim. How many bees are in it and are you seeing similar symptoms there?I foolishly bought the AFB test instead of the EFB test. I used that test and it was negative. I have an EFB test arriving Monday. When I went in I saw barely any larva to test. I saw barely any brood and no eggs. I did see the queen though.
Does it make sense to feed them? There is some honey and nectar in the hive. I feel bad just waiting and doing nothing.