> Do you preserve the bees prior to dissection or
> do they have to be fresh?
You can freeze them for long periods if you
don't want instant results. I sample into
ziplock bags and toss them in a small cooler
with plain old ice for all day, and then toss
the bags into a freezer when I am done for the
day. Bags are labeled with permanent marker,
but one wants to remember to do this before
collecting any bees.
> Do you just pull the head forward and the body
> backward with your fingers?
Yes, more with the fingernail than the finger.
One thing - you may never find any, depending
upon where you got your queens or what the
ancestry of your queen is. Tracheal mite
problems are a sign of needing a new queen
supplier more than anything, as only the
meven a feeble breeding and selection attempt
would include tracheal mite considerations.