If the screen will let the queens thru or even sting thru, there would indeed probably be one remaining before too long but that does not always happen. If you want to change the genetics of the mean hive, then kill the queen and after five days go in and kill all the emergency cells. Then take hopefully a light colored soft waxed frame of eggs and brood and put it without bees in the mean colony and let them raise a new queen off a milder mother. If you do this ten days below the tallow flow, it can result both in a new queen and a good big crop.