No, but it has a lot to do with the distance from the center of the brood nest. Bees build the smallest cells in the center of the brood nest and gradually increase in size as they go out. They will not exceed a certain size, but will vary with the brood area often composed of 4.9 or 5.0 cells while 3 or 4 frames from the center they draw 5.1 or 5.2. Also, cells will often be stretched a tad vertically and compressed horizontally. They may measure 4.9 one way and 5.1 the other. Check this by letting a colony build comb. It is very easy to demonstrate.Would any of this have anything to do with the frames sizes?