I do. My over-winter setup is a deep & medium.
I move the queen down into the deep (if need be).
Excluder is placed between the deep & medium.
Apiguard goes on top of the medium.
Bees usually have the gel out of the hive within a week for each treatment cycle.
I notice no disruption in queen laying.
Vance may be on the tater juice today already. He's referring to Amitraz and Formic Acid (I think) based treatments.
