i'll commend ya'll for that; but even the brits who have been doing scientific comparisons of those various interventions you list are quick to admit that once entrenched in an apiary mellisococcus plutonius is pretty hard to eradicate.
i missed early detection and quick intervention that's for sure. it's hard to quantify how much spreading to nearby hives occurred while i was waiting for a diagnosis.
the first two affected colonies were a little bit small coming out of winter compared to average, probably from doing a less than average job dealing with the high mite counts back during the previous fall brood up of winter bees. i chalked up these first two as having an issue with chilled brood, which was likely occuring after sharp drop in temperature.
heckfire, the oldtimers as well as the state apiarists here couldn't tell me the last time efb had been diagnosed anywhere near here, so i wasn't really thinking efb.
the irony is that coming out of winter is the time in the yearly cycle that i am going into my hives most frequently, as in at least once a week and sometimes more if splitting ect. and not just popping the top, but rather going through the broodnests to checkerboard and open up the nest and pyramid brood up to the next box ect.
over the course of just one to two brood cycless those beautiful solid brood patterns seen especially in the strongest colonies (2+ 10 frame deeps worth of bees) went from wall to wall solid capped brood to less than 25% still capped with the nonviable brood removed by the nurse bees. the open brood was also spotty with not very many old larvae, but rather mostly young larvae with a few of them and their jelly turning bright yellow.
i'll be spreading mine out and won't exceed 3 or 4 hives per yard with a least a mile or separation going forward. anything positive on the vita efb test will be considered hazardous waste and treated as such.