Yep, it makes a difference on how you handle them but then some bees are a lot easier to work with. My sons bees are far from the worst but they are a long ways from what mine are. He had quite a few one year that not only were quick on the draw but were runny and drippy. Forget about me ever finding a queen in there without a shaker box.
There are no ferals and very few surrounding bees where I am so it is not hard to keep mine quite true to to the breeders line. He continuously culls for gentle queens to graft from.