So three days ago I took five frames of various stages from 3 different 3 story Nuc's and started a 5 frame split. I have checked and checked for a queen. I ran them through a queen excluder, no queen. Yet, when I put a caged queen on the split, some, not all of the bees are aggressive to the cage trying to sting her. The other bee's display a typical surrounding her looking calmly in at her.
Now as an aside, when I first received her, she was piping. My research suggested that virgin pipe and that a queen less hive wont accept a virgin queen. I received the queen from a reputable source so I am not certain she a virgin.
My question is simply, is the split rejecting her? She is still in the cage so I can remove her if so.