I started OTS system this year, and as a result raise some queens. Well one of my hives the queen didn't mate, I found her 3 weeks after she should have taken her flight (4 from emerging), still small and a virgin. luckily I raised an extra queen in a mating nuc at the same time (they are sisters). She was laying beautiful patters I'm her nuc.
On the 8/8 I put her in a push in cage, and just put the whole nuce in the hive and smoked it like crazy figuring the nuc was mostly nurse bees, and the foragers would return to the other nuc I have put in its place that has a virgin queen. There wasn't a pile of dead bees in front of the hive the next day, so figured they were getting along. 8/10 (yesterday) I went to check, and they were either ignoring the cage, or trying to feed her. So I released her, watched on the frame and bees would run up to her, lick her and then run off.
Today I wanted to check to see how things were since I released her after only 2 days, I found her 2 frames over being chased and grabbed on to by about 4-6 bees. I knocked them off and her back in the push in cage figuring I went to fast.
Question is, should I have let them be? If she survived 24 hours, would they still get rid of her? Being my first time doing an intro, I didn't want to take a chance.
The nuc was put in the bottom box, so most of the old bees are in the top box. Should I put on a queen excluder to keep her in the bottom when I release her?
Thanks in advance!