DNA Testing on Honey Bees please
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  1. #1
    Join Date
    Jun 2015
    Location
    Yavapai County, Arizona, USA
    Posts
    64

    Default DNA Testing on Honey Bees please

    First I tried Google.
    Then I tried this site, searching for DNA and/or DNA test.

    Would someone please tell me where to send bees to get them DNA tested?

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  3. #2
    Join Date
    Apr 2016
    Location
    San Francisco, California, USA
    Posts
    13

    Default Re: DNA Testing on Honey Bees please

    What do you hope to learn by getting a DNA test? It seems there are no commercial companies specifically sequencing A. mellifera because there is virtually no utility to the end user except possibly in cases of commercial breeding programs. You might be able to determine some phylogenetic information about your bees by comparing to known lines like Africanized bees or Italian bees, but that's probably the most useful information you will get.

    If you are determined to get a genome sequenced I would try contacting a university doing this kind of research http://hymenopteragenome.org/beebase/?q=about There are a number of companies that will do whole exome sequencing (sequencing of the parts of the genome that actually express protein) this generally runs $500-$800 per sample and may require specialized sample prep or modified conditions for nonhuman samples. After all that you're going to receive a series of long files with raw sequence information that will be very difficult to interpret in a meaningful way. You may be able to say that you're sample differs from the reference sequence in certain regions but assigning a meaningful interpretation to that difference will be difficult.

  4. #3

    Default Re: DNA Testing on Honey Bees please

    Have you heard CD Genomics, which offers 16s, 18s rRNA and ITS Metagenomics Sequencing service by Illumina MiSeq Platform. Maybe you can get what you want there.

  5. #4
    Join Date
    Jun 2015
    Location
    Yavapai County, Arizona, USA
    Posts
    64

    Default Re: DNA Testing on Honey Bees please

    Thanks, I'll check it out...
    M

  6. #5
    Join Date
    Sep 2018
    Location
    Jacksonville Fl
    Posts
    26

    Default

    I know this is an old thread, but I am interested in finding a genetic test for my colonies + other local beeks.
    Is anyone aware of any new developments in this area? Trying to ID Russian vs Russian Hybrid colonies for example.

  7. #6
    Join Date
    Dec 2006
    Location
    St. Albans, Vermont
    Posts
    8,242

    Default Re: DNA Testing on Honey Bees please

    The Tarpy lab at North Carolina State does DNA testing

  8. #7
    Join Date
    Sep 2018
    Location
    Jacksonville Fl
    Posts
    26

    Default

    Perfect, thank you Michael!

  9. #8
    Join Date
    Jun 2018
    Location
    Boaz, KY, USA
    Posts
    2,018

    Default Re: DNA Testing on Honey Bees please

    Rhop:

    I had a similar question earlier in the year and received two (2) commercial options from Dr. Dainat with the Swiss Bee Lab:

    https://apigenix.com/

    "This company was specialized in microsatellites. They are in the process to also have the SNP approach, coming soon. Anyway methods apart, the purpose was to determine the type of bees you have i.e. for example ligustica or whatever, and calculate hybridization (i.e. how “pure” is your bee. Of course there is to keep in mind that pure honeybee does not exist and have never existed. Probably if you have 10 % hybridization it is already very good. With a good breeding program, you can aim at less).

    There is another company in Switzerland, where SNP is established: https://www.mp-genetics.com/ also to see honeybee subspecies, type etc…"

    Not sure what shipping to Switzerland looks like, but it appears that this type of testing is becoming more mainstream with each passing year.

    Russ
    Ecclesiastes 11:4

  10. #9
    Join Date
    Sep 2018
    Location
    Jacksonville Fl
    Posts
    26

    Default

    Truly appreciate the advice Russ

  11. #10
    Join Date
    Feb 2001
    Location
    Enfield,Ct.
    Posts
    735

    Default Re: DNA Testing on Honey Bees please

    Contact someone in the Rus Breeders Org.
    The test their own bees annually.

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