16S rRNA Amplicon Sequencing Demonstrates that Indoor-Reared Bumblebees (Bombus terre
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  1. #1
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    Default 16S rRNA Amplicon Sequencing Demonstrates that Indoor-Reared Bumblebees (Bombus terre

    16S rRNA Amplicon Sequencing Demonstrates that Indoor-Reared Bumblebees (Bombus terrestris) Harbor a Core Subset of Bacteria Normally Associated with the Wild Host
    A MiSeq multiplexed 16S rRNA amplicon sequencing of the gut microbiota of wild and indoor-reared Bombus terrestris (bumblebees) confirmed the presence of a core set of bacteria, which consisted of Neisseriaceae (Snodgrassella), Orbaceae (Gilliamella), Lactobacillaceae (Lactobacillus), and Bifidobacteriaceae (Bifidobacterium). In wild B. terrestris we detected several non-core bacteria having a more variable prevalence. Although Enterobacteriaceae are unreported by non next-generation sequencing studies, it can become a dominant gut resident. Furthermore the presence of some non-core lactobacilli were associated with the relative abundance of bifidobacteria. This association was not observed in indoor-reared bumblebees lacking the non-core bacteria, but having a more standardized microbiota compared to their wild counterparts. The impact of the bottleneck microbiota of indoor-reared bumblebees when they are used in the field for pollination purpose is discussed.
    http://journals.plos.org/plosone/art...l.pone.0125152
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  3. #2
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    Default Re: 16S rRNA Amplicon Sequencing Demonstrates that Indoor-Reared Bumblebees (Bombus t

    So... if the bumble bee is kept inside, it gets one kind of bacteria, but if we let it roam outside it also gets other bacteria.

    I am trying to grasp the value of this research.

  4. #3
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    Default Re: 16S rRNA Amplicon Sequencing Demonstrates that Indoor-Reared Bumblebees (Bombus t

    The point is even isolated bumblebees have some of the bacteria, but bumblebees in their native environment have a diversity. Diversity is good in this instance also.
    americasbeekeeper.com
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