Instrumental Insemination Equipment - Page 4
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  1. #61
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    Default Re: Instrumental Insemination Equipment

    Quote Originally Posted by Little boy blue View Post
    Do you use the gear yourself?
    Yes, I have a friend in Ireland who makes the ruby sting hooks for me.

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  3. #62
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    Default Re: Instrumental Insemination Equipment

    Quote Originally Posted by Little boy blue View Post
    Thank you, The syringe is half an inch diameter and internal volume is two and a half inch stroke, I plant to use it independently of saline and as a vacuum pump only.

    How much sperm does the average drone give?

    Do you have any experience with cryogenic storage of bee semen?
    If the syringe is for insemination work, it sounds too coarse. Air will not work, it needs to be a fluid filled hydraulic system as the semen is very viscous and the movements are tiny. The average drone produces about 0.75-1.0 microliters of semen.

    Cryopreservation is not very practical for the average inseminator.

    0.2 is the upper limit for sting hooks. I prefer to go a little smaller, even down to about 0.16. I don’t use them any more… but when I did, I like to grab the sting closer to the tip.
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  4. #63
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    Default Re: Instrumental Insemination Equipment

    One of my first instruments about 25 or so years ago.
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  5. #64

    Default Re: Instrumental Insemination Equipment

    Quote Originally Posted by beekuk View Post
    Yes, I have a friend in Ireland who makes the ruby sting hooks for me.
    Hi

    I have located some rubies and see what they mean about them guiding the sting in, they have a small dimple at the centre, here is what I have found in case others might like to have a go, I will opt for the 0.2 hole and a 3.00 mm outer as I can handle that size I think.

    I looked at the watch movements I have and they are way too small, can hardly see them.

    https://www.cousinsuk.com/category/jewels-seitz

    It's gonna be fun for sure, but I will give it a go at three for twelve pounds ain't bad.

  6. #65

    Default Re: Instrumental Insemination Equipment

    Hi JSL

    Cool set up.

    I Was thinking of something similar for the needle holder when I used Holly for the body, but by using brass tube to take the needle or as the glass boys tend to call a spindle, would you say with a steady hand that one could line things up without a micrometer setup?

    Got you on the fluid hydraulic model, on one of the videos it shows someone pulling the semen with only a small amount of saline above the main body of semen, maybe this is where I got swayed off of course.

    I have the nylon screws on the way and will fit them as part of the final prototype just in case I think.

    I'm all questions at the moment.

    Is it absolutely necessary to filter the saline through the 0.2 filter to sterilise it before drawing the samples.

  7. #66
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    Default Re: Instrumental Insemination Equipment

    Quote Originally Posted by Little boy blue View Post
    Hi

    I will opt for the 0.2 hole and a 3.00 mm outer as I can handle that size I think
    3mm is way too big as you will have to set the ruby in a hook. I do not care for the ruby hooks as they are bigger and clunkier than I like. I used to make perforated sting hooks out of brass and they worked just fine. You can actually make the hole with a fine sewing needle or better yet buy a micro drill bit. Below is a brass hook "drilled" with a sewing needle.
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  8. #67
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    Default Re: Instrumental Insemination Equipment

    Quote Originally Posted by Little boy blue View Post
    Hi JSL


    would you say with a steady hand that one could line things up without a micrometer setup?

    Got you on the fluid hydraulic model, on one of the videos it shows someone pulling the semen with only a small amount of saline above the main body of semen, maybe this is where I got swayed off of course.


    Is it absolutely necessary to filter the saline through the 0.2 filter to sterilise it before drawing the samples.
    Yes, it is possible to even “free hand” inseminate. It takes a little more skill and a lot less coffee.

    The wooden instrument I posted in similar to what you describe as the syringe is manually positioned. It just slides in the block.

    You can heat sterilize the saline. Just start clean and keep it clean.
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  9. #68

    Default Re: Instrumental Insemination Equipment

    Great idea of using a sewing needle for a drill, would save money buying the tiny ready made ones from the hobby shop.

    I have looked through her indoors sewing box and the very finest needles are all half a millimetre, to recap, am I correct in saying that the hole is 20" thou or 0.50mm half a millimetre.

    I must say this is a great place to join for information and the hospitality is very warm and welcoming indeed, thank so much thus far.
    Last edited by Barry; 01-14-2017 at 03:19 AM.

  10. #69
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    Default Re: Instrumental Insemination Equipment

    Just use the very tip of the sewing needle or sharpen one if it is a little too blunt. It takes a little practice, but you do not want to pass it through the hook, just the tip. I showed this to a friend of mine that was a a very skilled machinist and he just laughed and said I had way too much time on my hands.
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  11. #70
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    Default Re: Instrumental Insemination Equipment

    Where are the other honey bee inseminators?
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  12. #71
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    Default Re: Instrumental Insemination Equipment

    They all got kicked out and died before winter....
    Last edited by Barry; 01-14-2017 at 03:20 AM.

  13. #72
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    Default Re: Instrumental Insemination Equipment

    Quote Originally Posted by JSL View Post
    Where are the other honey bee inseminators?
    Joe... do you also make your own forceps?

  14. #73

    Default Re: Instrumental Insemination Equipment

    Quote Originally Posted by JSL View Post
    Just use the very tip of the sewing needle or sharpen one if it is a little too blunt. It takes a little practice, but you do not want to pass it through the hook, just the tip. I showed this to a friend of mine that was a a very skilled machinist and he just laughed and said I had way too much time on my hands.
    Ahah, right got you.

    This is going to leave a slightly tapered hole in the sting hook, that will match the taper of the sting itself, the more one pushes the needle inwards the larger the hole and taper will be.

    I have some nice one millimetre nickel silver or Sterling silver that will make the hooks and will be easier to drill, I will give it a go tomorrow.

    Cheers guys.

  15. #74

    Default Re: Instrumental Insemination Equipment

    Quote Originally Posted by JRG13 View Post
    They all got kicked out and died before winter....
    Like the answer, we human males don't know how lucky we are, lol.

  16. #75
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    Default Re: Instrumental Insemination Equipment

    Quote Originally Posted by JRG13 View Post
    They all got kicked out and died before winter....
    lol, I should have clarified...
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  17. #76
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    Default Re: Instrumental Insemination Equipment

    Quote Originally Posted by beekuk View Post
    Joe... do you also make your own forceps?
    No, the Swiss and Germans are much better at making quality forceps.
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  18. #77

    Default Re: Instrumental Insemination Equipment

    I was thinking today and wondered why nobody has not made a loope type noose sting grabber yet, ot maybe they have, you know one that lassoo's the sting then pulls tight.

    I thought of something like a bee shaped point that is pulled back under spring tension onto a matching surface.
    Last edited by Barry; 01-14-2017 at 03:20 AM.

  19. #78
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    Default Re: Instrumental Insemination Equipment

    This is a slow business, slowly have to get the goods together so I will have to refer to this thread as I progress 1 step at a time. Just keep the ideas coming, I will get up to my nearest Hobby Lobby and see if they have any brass tubing available O otherwise I will get some in from the site you recommended although they state 1/4" is out of stock, I also have a spare microscope slide adjuster I could try to use to lower the syringe.
    Johno

  20. #79

    Default Re: Instrumental Insemination Equipment

    I gathered for about three weeks or so before making something, doodled in those weeks, but after joining the forum the blast of information from willing participants really got Casey Jones's engine No 1 rolling.

    My grandfather was right all those years ago, his motto was, help those who want to help themselves, and if a few were struggling along, he would say, don't tell me, show me, and this is exactly what has happened here on Beesource.

    I know after talking with Pete, this is no easy task, highly specialised and well worth the savings if one can bring the fruits to bare. Here we are on a very limited budget and have to watch every dime, so getting things working is a bonus for us here in the sticks, where postage can cost as much as some of the items.

    What Beesource has in its data bank, is hand to eye coordination, experience, which are worth far more than money ever could be, the most valuable of all is our own labour, this is why governments think we owe them a living.
    Last edited by Barry; 01-14-2017 at 03:20 AM.

  21. #80
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    Default Re: Instrumental Insemination Equipment

    Quote Originally Posted by Little boy blue View Post
    I was thinking today and wondered why nobody has not made a loope type noose sting grabber yet, ot maybe they have, you know one that lassoo's the sting then pulls tight.

    I thought of something like a bee shaped point that is pulled back under spring tension onto a matching surface.
    Check out some fine threaders for fishing. http://www.bing.com/images/search?q=...fo0&ajaxhist=0

    They're pretty fine, they're made to pass through the eye of small hooks, then you pass the leader through and pull it through to thread the eye. I'm betting it could catch the sting though and maybe hold it in place with just tension or slip a very small O-ring on and slide it forward to secure but you'd have to be very careful.

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