Instrumental Insemination Equipment - Page 3
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  1. #41

    Default Re: Instrumental Insemination Equipment

    Hi JSL

    I Caught that article earlier on your URL GREAT READ and lots of ideas in there, liked the part where it mentioned freedom of movement for opening the vaginal flap by having more freedom doing it manually, this is what gave me the idea of a free floating needle movement, which my next prototype will enable.

    I have done some very fine work in the path labs this way entirely by hand so I know it can be done, the bee model is a little easier to achieve because your dealing with millimetres instead of thousands of an inch like I was doing.

    The idea is to have a fully supported and hanging needle and dampened, so that it can be moved entirely by hand without all the graduated rack and pinion set up, but have depth stops so you cannot go in too deep, I will build one pretty soon to see how it performs.

    I know it can be done because I have used similar devices in the path lab, some of which we made ourselves in the early days, I have lots of ideas, and now the latest 3D software to draw it out first as I normally do today, to save wasting time and materials, Solidworks is the engine of choice today, what you see on the screen is what you get in reality.

    Thanks again.
    Last edited by Barry; 01-14-2017 at 03:21 AM.

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  3. #42

    Default Re: Instrumental Insemination Equipment


  4. #43
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    Sep 2007
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    New Albany, Ohio, USA
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    Default Re: Instrumental Insemination Equipment

    No, those are not the articles I was thinking of. Laidlaw and Goss wrote an ABJ article describing a simple, mostly off the shelf instrument using rod-end ball joints. However, the link above that you shared has an image in it that is of a very rare Laidlaw Instrument. There were only a handful ever made, about 7 I think at a cost of about $3000 each at the time. All movement were controlled by micro manipulators.
    Last edited by Barry; 01-14-2017 at 03:21 AM.
    Breeder Queens & Honey Bee Nutritional Supplements
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  5. #44
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    Default Re: Instrumental Insemination Equipment

    Breeder Queens & Honey Bee Nutritional Supplements
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  6. #45

    Default Re: Instrumental Insemination Equipment

    I see it has a metal version of the Harpo pump, looks very complicated like the others but it will need a bit better finish to the components as deep scratches can harbour bacteria.
    Last edited by Barry; 01-14-2017 at 03:22 AM.

  7. #46

    Default Re: Instrumental Insemination Equipment

    I have made a few amendment to the second prototype.

    I have found a better way of securing the outer slider housings for the two internal sliding pin vices, I filed two small slots using a jewellers needle file for two rubber O rings to locate the tube either side of the ball joint, these also act as dampeners to the pin vice slide with just the right amount of grip to prevent things for moving, the feel is really good.

    These tiny pin vices are idea as it allows for multiple hooks and guides to be made and removed for cleaning, I have seen some pretty dirty tools on several videos when they are fixed ends, at the prices these cost you can have several to hand ready made for a quick change over if required, simply slide them in and out.

    https://s29.postimg.org/7wucndr47/IMG_0081.jpg

    https://s23.postimg.org/me7acx4ej/IMG_0084.jpg

    https://s23.postimg.org/54as87knf/IMG_0082.jpg

    And a newer base made from quality birch ply 3/4" and glued a PB 102 bronze plate to the top using contact adhesive, JSL rightly point out that this will need to be wiped down with alcohol etc, bronze has good antibacterial properties and is tougher and more scratch resistant than copper.

    The base may need rubber feet under it to lift above some scopes base plates.

    https://s24.postimg.org/u370vtkol/IMG_0087.jpg

    https://s30.postimg.org/cv2bw4d1t/IMG_0085.jpg

    The hands can comfortably circumnavigate the tool post like before, thus keeping a good hold of the base as you work under the scope.

    https://s30.postimg.org/vhtft4xox/IMG_0086.jpg

    One thing I learned using scopes is, if your illuminating a white subcutaneous substances like the queen bees nether regions, it's sometimes best to use a coloured light source, as this shows up the delicate folds within, different colours show different attributes etc.

    The queen post will be fully adjustable in X Y Z Planes, upon a locking ball gimble like a camera tripod head, but once you find the optimum height for working you won't need to adjust things as much, but the tool posts can be adjusted for height and angle if required, via the full inch of thread within the rod end body, this eliminates clutter and cost of the overall set up.

    Plenty of ideas here,

    http://www.bearingboys.co.uk/Ball-Joints-3720-c

    http://www.springfixlinkages.com/en/...t-joints/r3540

    This may be my final version to use, now for the next stage and the fully floating injection apparatus.
    Last edited by Little boy blue; 12-17-2016 at 09:33 AM.

  8. #47

    Default Re: Instrumental Insemination Equipment

    This is not bee related but where I got the idea of the spring adjuster from. It's a leather strap cutter that I made several years ago, the ergonomics might give folks a few more ideas in how they decide to make their versions of tools or tooling.

    https://s23.postimg.org/shwzmx7i3/IMG_0088.jpg

    https://s28.postimg.org/x0cz98ncd/IMG_0089.jpg

    https://s24.postimg.org/8c7jkk9hx/IMG_0090.jpg

    All brass body and cam lock adjustment, handle in Hazel from our farm.

    Thanks for looking.

  9. #48
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    Default Re: Instrumental Insemination Equipment

    Coming along nicely! Do the rod ends have much tension on the ball inside? In your pics, the "hooks" are tilted down. I wasn't sure if that is just the way you positioned them or if they drift that way? The instruments I made with rod-end ball joints needed a little tension on the ball joints. It is a delicate process to tap and thread one of those without messing up the smooth surface and movement. I used nylon thumb screws to put tension/pressure on the ball.
    Breeder Queens & Honey Bee Nutritional Supplements
    www.latshawapiaries.com

  10. #49

    Default Re: Instrumental Insemination Equipment

    Hi JSL

    The ball joints are quite tight and can easily support the arms at full length and you still have to push them down, these should last a good few years before they need either some thick spray grease or. If you look at the picture of how the nylon rod ends are made, you will see the injection hole where they are filled when they are injection mounded, this can be drilled and tapped out to an 1/8" and a nylon screw fitted to tighten things up, but I don't think they will need it for quite a while.

    To do this, remove all the fittings, turn the ball centre towards the injection hole so it doesn't mark the surface before you drill and tap. I may do this at some stage but only if needed.

    https://s28.postimg.org/hi1v3qavx/IMG_0095.jpg

    https://s27.postimg.org/6kie5kaqb/IMG_0092.jpg

    I found the ideal kit for the queen post, I borrowed one of my surface gauge clamps for now, the tubes are both 11/32" and 1/4" respectfully, I will turn some wooden bodies to house the queens, this way I have more control over internal sizing.

    https://s29.postimg.org/6vyqtj2if/IMG_0094.jpg

    https://s27.postimg.org/dvf8lyi9v/IMG_0091.jpg

    For the gas I can insert an angled connector into the queen post tube underneath.

    Forgot to mention this earlier, "do not !" cut the tube using a pipe cutter or it will compress the wall and you will have a lot of work getting the tubes to slide inside each other, better to use a fine metal saw or jewlerers file and work your way around carefully.
    Last edited by Barry; 01-14-2017 at 03:22 AM.

  11. #50
    Join Date
    Dec 2011
    Location
    Lottsburg, Virginia USA
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    Default Re: Instrumental Insemination Equipment

    I have ordered some mini pin chucks and will order ball joint bearing ends from Amazon, would it be an advantage to have the microscope fixed directly to the base? If I bolted a 12" piece of 1" round bar to the base the microscope could come off its stand and go onto the base. Once I have the bearing ends the next job would be to locate some brass tubing to suite, so the saga begins.
    Johno

  12. #51

    Default Re: Instrumental Insemination Equipment

    Here is a place that does tubing.

    http://www.hobbylinc.com/brass-hobby...t-metal-tubing

    The brand is K & S tubing USA

    http://ksmetals.com

    .
    Last edited by Barry; 01-14-2017 at 03:22 AM.

  13. #52

    Default Re: Instrumental Insemination Equipment

    Here is my home made Harbo style pump, from the scrap box, two water pipe bungs turned down to fit a small section of K & S tubing which luckily fit over the ends perfectly.
    I hand turned them in the wood lathe as I normall do, using the standard HSS gouges, which is a pleasure to do when working in brass.

    https://s27.postimg.org/u0ofq0lib/IMG_0097.jpg

    https://s30.postimg.org/tgxpcgbpd/IMG_0100.jpg

    https://s27.postimg.org/kz8z9gn2r/IMG_0103.jpg

    https://s29.postimg.org/51s58kp53/IMG_0104.jpg

    She works a treat with a very gentle draw rate, cosy including the o ring about a Dollar.
    Last edited by Little boy blue; 12-18-2016 at 03:20 PM.

  14. #53

    Default Re: Instrumental Insemination Equipment


  15. #54

    Default Re: Instrumental Insemination Equipment

    Queen intro cage, made today.

    https://s23.postimg.org/uj2li73m3/IMG_0108.jpg

  16. #55

    Default Re: Instrumental Insemination Equipment

    Quote Originally Posted by johno View Post
    I have ordered some mini pin chucks and will order ball joint bearing ends from Amazon, would it be an advantage to have the microscope fixed directly to the base? If I bolted a 12" piece of 1" round bar to the base the microscope could come off its stand and go onto the base. Once I have the bearing ends the next job would be to locate some brass tubing to suite, so the saga begins.
    Johno
    Johno

    Here is a viable unit for adaptation for the syringe needle mount ete.

    https://www.amazon.com/OMAX-Mechanic.../dp/B00FG88P3A

    Some simple x y stages here, not too expensive.

    https://www.thorlabs.com/newgrouppag...tgroup_id=2952
    Last edited by Little boy blue; 12-18-2016 at 05:43 PM.

  17. #56
    Join Date
    Sep 2007
    Location
    New Albany, Ohio, USA
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    Default Re: Instrumental Insemination Equipment

    Danny,

    The brass is beautiful, especially with the finish you have on the pieces! A word of caution though as the saline solution tends to corrode brass pieces. Also, it is hard for me to gauge the size of the syringe, but a good displacement volume is 8-12 microliters per revolution of the syringe knob. This will give you reasonable control.

    Keep up the good work!
    Breeder Queens & Honey Bee Nutritional Supplements
    www.latshawapiaries.com

  18. #57

    Default Re: Instrumental Insemination Equipment

    Thank you, The syringe is half an inch diameter and internal volume is two and a half inch stroke, I plant to use it independently of saline and as a vacuum pump only.

    How much sperm does the average drone give?

    Do you have any experience with cryogenic storage of bee semen?

    I get the finish on the old buffer and the lathe the old fashioned way.

    I have another couple of simpler ideas coming soon.

    Thanks.
    Last edited by Barry; 01-14-2017 at 03:23 AM.

  19. #58

    Default Re: Instrumental Insemination Equipment

    Please could anyone advise what size hole best used for the sting hook on the insemination tool that I'm making.

    Thanks.

  20. #59
    Join Date
    Dec 2008
    Location
    SOMERSET, ENGLAND
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    461

    Default Re: Instrumental Insemination Equipment

    Most supplied are with 0.2mm hole size.

    I prefer to use a ruby sting hook, which you could make yourself.
    Last edited by Barry; 01-14-2017 at 03:23 AM.

  21. #60

    Default Re: Instrumental Insemination Equipment

    Thanks Pete

    I was wondering if I could use a jewel out of some old watch movements I have kicking about somewhere, does it have to be exactly 0.2 or can you get away with plus or minus a bit.

    I know I don't have drills that small, so the jewel might be slightly bigger diameter, I have watched a couple of videos on YouTube and one said the jewel ruby helps getting the sting through the middle.

    Do you use the gear you made yourself?

    Dan.
    Last edited by Barry; 01-14-2017 at 03:23 AM.

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