Instrumental Insemination Equipment - Page 2
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  1. #21
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    Dec 2011
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    Lottsburg, Virginia USA
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    Default Re: Instrumental Insemination Equipment

    I have obtained a Nikon stereo zoom 15x eyepieces by 0.8 to 4 zoom which gives me 12 times magnification up to 60 times. I have heard some talking about fitting a Barlow lens to increase focal depth but will have to see how this will work. Any further ideas are really appreciated.
    Johno

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  3. #22
    Join Date
    Sep 2007
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    New Albany, Ohio, USA
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    Default Re: Instrumental Insemination Equipment

    Quote Originally Posted by Little boy blue View Post

    The tip is less than 1.00mm, my idea is to get one which will act as tube and needle in one section.

    https://s30.postimg.org/s3pvmbj01/IMG_0060.jpg

    There are several cheaper end stereo scopes but they all tend to start at 20 times mag, do you know of any other affordable units less than 200.
    It looks nice, but that one is going to hurt. The tips are so fine and delicate, you may be better off pulling them out of a smaller material.

    I donít know of any scopes off hand that come in under 200. You may have to keep your eyes open for a used scope on the internet.
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  4. #23
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    Sep 2007
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    Default Re: Instrumental Insemination Equipment

    Quote Originally Posted by Little boy blue View Post
    I made these two brass smokers a few years ago to an old English design, they work pretty well for the quick inspection, the fuel lasts about fifteen to twenty minutes.

    https://s19.postimg.org/dm8xcljo3/20160923_092400.jpg
    Beautiful!
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  5. #24
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    Default Re: Instrumental Insemination Equipment

    Quote Originally Posted by johno View Post
    I have obtained a Nikon stereo zoom 15x eyepieces by 0.8 to 4 zoom which gives me 12 times magnification up to 60 times. I have heard some talking about fitting a Barlow lens to increase focal depth but will have to see how this will work. Any further ideas are really appreciated.
    Johno
    That should be perfect. Nikon scopes have nice optics!
    Breeder Queens & Honey Bee Nutritional Supplements
    www.latshawapiaries.com

  6. #25

    Default Re: Instrumental Insemination Equipment

    Quote Originally Posted by JSL View Post
    It looks nice, but that one is going to hurt. The tips are so fine and delicate, you may be better off pulling them out of a smaller material.

    I donít know of any scopes off hand that come in under 200. You may have to keep your eyes open for a used scope on the internet.
    I was thinking the points might be on the small side, I will try a little more heat when I get the replacement cyclinders after the new year and have a go then, I will buy some two millimetre rods next, but if I can get the 4.0 to work it a bonus for what I have in mind.

    Been busy in the shop today and things are moving along nicely.

    Thanks.

  7. #26
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    Sep 2007
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    Default Re: Instrumental Insemination Equipment


    This is one of the first syringes I made. It is the fine adjustment guts from a microscope and a 1cc glass syringe complete with a leather plunger seal and cherry wood barrel. This is old school!
    Attached Images Attached Images
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  8. #27

    Default Re: Instrumental Insemination Equipment

    Please could you advise the best dia spindle tip to acquire after drawing out.
    Last edited by Barry; 01-14-2017 at 03:24 AM.

  9. #28
    Join Date
    May 2012
    Location
    Sacramento, CA, USA
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    5,457

    Default Re: Instrumental Insemination Equipment

    regarding the pipette,

    You could pull one fine enough if you're careful from that. I was aspirating day old larva with one I made this year and I didn't have to pull that one very small.

  10. #29
    Join Date
    Sep 2007
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    New Albany, Ohio, USA
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    Default Re: Instrumental Insemination Equipment

    Quote Originally Posted by Little boy blue View Post
    I was thinking the points might be on the small side, I will try a little more heat when I get the replacement cyclinders after the new year and have a go then, I will buy some two millimetre rods next, but if I can get the 4.0 to work it a bonus for what I have in mind.

    Been busy in the shop today and things are moving along nicely.

    Thanks.
    To give you an idea, I use a tip opening, ID, of about 0.008” or 0.20mm give or take. I normally start with 1.2-1.4mm glass pipettes for pulling.
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  11. #30

    Default Re: Instrumental Insemination Equipment

    The spindle I made today is only half a millimetre I/D but with a little practice I should be able to get the desired diameters, it was a lack of heat that prevented the tip from drawing out properly, it has to be right I know so more playing until it's optimum.

    The girl on the link I put up earlier made it look so easy and she was using much thinner gauge tube and double drawing it, very experienced operator indeed.

    I have found a novel way of cutting the tubes, I use an old diamond coated router cutter and roll it on the edge of it, snaps really clean when it's scored.

    I noticed that some ends are fired gently after cutting to round over the very edges of the tip, will have to try this too, it will save on deburrring later.
    Last edited by Barry; 01-14-2017 at 03:24 AM.

  12. #31

    Default Re: Instrumental Insemination Equipment

    Here is my project so far, I will give as accurate step by step tutorial as possible, and edit as I go so nothing gets lost.

    Here are the basic parts.

    https://s24.postimg.org/on07pznj9/IMG_0062.jpg

    https://s23.postimg.org/7eznesc9n/IMG_0068.jpg

    Here one has two 1/4" bore rods ends, nylon insert.

    http://www.bearingboys.co.uk/Rod-End...earing-88403-p

    The next item is the needle body, which consists of a wooden body made from English holly, a section of 1/2" wide brass strip 5/64 thick, which fit into a slot cut so they sit flush, two nickel silver, or they can be made from brass, pivots are hand turned on the wood lathe using HSS wood turning tools, the old fashioned way using hand turning skills.

    One pivot is drilled with a 4.00 mm hole the other tapped out to 4.00 mm, there is a two inch 4.00 mm all thread bolt and spring and the brass axle and two self washer 5.00 mm nuts.

    https://s27.postimg.org/4ms9kosrn/IMG_0071.jpg

    One can comfortably reach around each post to manipulate the tools into place.

    https://s28.postimg.org/7gbaqth0t/IMG_0079.jpg

    The rod ends are threaded onto a brass rod 1/4 inch diameter and tapped firmly into a pilot in the wooden base, to get them tight so they don't move drill the last half in a size below or you can glue them in, I prefer the former so you can remove them from below using a drift if needed.
    The rod end post also have a full inch thread so there is ample range for vertical adjustment if needed.

    The rod ends have a three inch 1/4" tube fitted through the pivot bearing and are secured via a piece of shrink wrap either side, then the 7/32" tube with its pin vice slide through that, to get the desired amount of resistance on the outer tube all you need to do is very gently tap the 1/4" tube to dissform it until you have slight interference fit, do this a tiny bit at a time until you have the desired damping needed.

    1 X 1/4" ' and 1 X 7/32 brass tube are from K & S tubing from the United States, most good model shops carry this as standard, their wall thickness is 1/64 and they fit inside each other perfectly.

    Inside the 7/32" brass tube fit two mini pin chucks perfectly, you can glue them in if you like using a tiny dot of super glue, and to remove it again afterwards all you need is a little hear from a cigarette lighter and the glue will melt again, here is where I got mine from.

    http://www.rnhorological.co.uk/image...-S232%20RN.jpg

    And here is where I got the brass tubing.

    https://www.phillsmodels.com

    Showing the needle post, not yet fully complete with its pump or adjuster, to follow next.

    https://s24.postimg.org/o6a4sshs5/IMG_0077.jpg


    https://s28.postimg.org/5ew7ves31/IMG_0078.jpg


    https://s23.postimg.org/fjhib3q6z/IMG_0076.jpg

    The pivots can be moved well out of the way to place the queen and tube into place, the apparatus for this will also be unique and multi adjustable for ease of use, to follow also.

    https://s24.postimg.org/g6ygyzthx/IMG_0080.jpg

    This is an able prototype I think and inexpensive for anyone to have a go at making one.

    I'm already working on the next one which will be based on this one but with a unique feed in system for the injection, that anyone will be able to handle.

    Everything sits on top of the Ash wooden base covered with a nice strip of polished leather for comfort, the hands can also be rested upon the board making everything very secure.

    Thanks for looking, questions please, ideas definitely.
    Last edited by Little boy blue; 12-16-2016 at 04:58 PM.

  13. #32
    Join Date
    Dec 2011
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    Lottsburg, Virginia USA
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    Default Re: Instrumental Insemination Equipment

    Little Boy Blue, I am impressed. I have the microscope but that is as far as I have got. I will be 73 soon and have felt that spending a large amount of money on the equipment at my age would not give me a reasonable return on the investment. Some times I sits and thinks and sometimes I just sits, so seeing what you have accomplished will now stop me from just sitting and start me thinking. I will follow your postings on this subject with interest and see if I can add something in the future.
    Johno

  14. #33

    Default Re: Instrumental Insemination Equipment

    Thanks Johno

    I'm not quite sixty yet but beginning to feel like it when we are in the yard or garden allotment digging.

    You can get the parts at your side of the pond for sure, and most parts bar a couple are in imperial sizes.

    For your base, make it an 18 inch triangle, with rounded corners, not a long one like the one shown, so you can fit your microscope in a hole at the centre and the posts at either side, I have a scope in mind that can be mounted to this board using just the main post, I'm sure you can see where this is headed.

    I got the idea from lab equipment in my medical years so I know what to aim for, but all will be revealed as they say.

    I'm also having some 3D printed queen bee training models made that I have drawn up in Solidworks, so you can practice out of season and not on live specimens or wasting gas at the very beginning.

    If your going to follow wait until I show the next model, but do make the posts and arms, pin vices etc as these will be added to the new model.

    I'm not doing this as a commercial enterprise but will be copyrighting certain items as I go, thus self copyrighting the design to stop people from monopolising it at a later date commercially, but everything is free to who ever, in the words of my grandfather, we must whenever possible help those who want to help themselves.

    Respect

    Danny.
    Last edited by Barry; 01-14-2017 at 03:24 AM.

  15. #34
    Join Date
    Mar 2015
    Location
    Gadsden, AL, USA
    Posts
    83

    Default Re: Instrumental Insemination Equipment

    I was looking to try something along this line for this spring. I seen one other self made stand, that is similar in style to yours. It is helpful to have the details you are posting.

    Thanks for the posts.

  16. #35

    Default Re: Instrumental Insemination Equipment

    Hi RB

    Did you by any chance keep some pictures of the other home grown idea?
    Last edited by Barry; 01-14-2017 at 03:25 AM.

  17. #36
    Join Date
    Sep 2016
    Location
    Windsor, ON
    Posts
    8

    Default Re: Instrumental Insemination Equipment

    very interesting topic
    do you see the possibility of using a cell phone with a macro lens, instead of the microscope
    http://www.walmart.ca/en/ip/mercury-...=richrelevance, whether it is just a sick imagination of the old man?

  18. #37

    Default Re: Instrumental Insemination Equipment

    Hi al

    That's a cool idea, just watched a few tests on the tube and it looks like it would work, if you could use the phones camera to do the looking.

    I tried my Samsung J 3 just now and it focuses down to around and inch or so but hunts for focus, I wonder if you can blue tooth to a larger screen like you lap top to get a view that way.

    Thanks for the heads up, cheers.

  19. #38
    Join Date
    Sep 2016
    Location
    Windsor, ON
    Posts
    8

    Default Re: Instrumental Insemination Equipment

    thought of such lenses to celphone
    https://www.aliexpress.com/wholesale...20161216163516
    and the linc of the previous post is to serve as a stereoscope, I do not know whether it is clear what I invented

  20. #39

    Default Re: Instrumental Insemination Equipment

    Something really cool if it works instead of a microscope.

    Check out the quality visuals.

    https://m.youtube.com/watch?v=oUyzf45j-2M

    Now all we need is one that will focus close and let you work under it as well.

    Maybe

    https://m.youtube.com/watch?v=Fbi6L3GEj9Q
    Last edited by Barry; 01-14-2017 at 03:25 AM.

  21. #40
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    Sep 2007
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    New Albany, Ohio, USA
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    Default Re: Instrumental Insemination Equipment

    Danny,

    The prototype is looking nice! I like your craftsmanship. One word of caution though is leather may not be a surface of choice. It is nice to wipe things down with alcohol and I am not sure how well the leather will hold up over time.

    Your instrument looks like a Laidlaw-Goss (I think it was Goss) instrument published in the bee journals many years back. I have one I built around here some place. I need to dig it out and get a pic!

    Another interesting read.

    https://hal.archives-ouvertes.fr/hal-00891146/document

    Graham/Rader, if you are watching, do you know where to find a PDF of this article below?

    Laidlaw-Goss queen bee pre-set artificial insemination instrument.

    Journal Title: American bee journal.
    Journal Volume/Issue: Nov 1990. v. 130 (11)
    Main Author: Laidlaw, H.H. Jr.
    Other Authors: Goss, J.R.
    Format: Article
    Language: English
    Subjects: queen honey bees
    Apis mellifera
    artificial insemination
    equipment
    purchasing
    United States
    For More Info: View in NAL's Catalog.
    Breeder Queens & Honey Bee Nutritional Supplements
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