Well, normally I just stuck a box with foundation on top of the hot box with an excluder in between - then smoked or stuck a piece of cardboard with stinky-stuff on it in the hive entrance. I suppose up or down wouldn't matter. I just did it that way because I exposed my self to them less. It was easier to toss a box on top. It's kind of hit or miss though - half the time they just fly off or hang on the side - and a queen if she really wants to, can squeeze through an excluder. I have also taken them and shaken them into an empty box using an excluder to sift for the queen - I actually do it pretty often. It's not for the faint of heart though because bees go everywhere, and you better be suited up good!