Results 1 to 20 of 31

Hybrid View

  1. #1
    Join Date
    Mar 2014
    Location
    Lembach, Austria
    Posts
    13

    Default Job transgenic bee

    Hi!

    I am making a bee that is resistant to everything. Only problem is that I am a biotechnologist and have no experience with beekeeping.
    Is there someone willing to help? He / she needs experience in insemination and collecting a drone's sprerm.

    I could pay him/her, for doing the insemination for me If possible, you could come to Ireland for three months (I pay the flights).

    We make it happen before Monsanto does! Maybe we patent it, but we will never sue anyone if the genetic material mixes. I hate that practice!

    As a sidenote for the anti-gmo people: It is totally harmless for both the environment and for the bees. No bee is hurt! We are working tightly together with EPA and will never release a bee until we get approval to do so.
    (Inserted DNA is not dangerous in any way - it's just e.g. if a pesticide resistance is inserted into a plant, the farmer then uses the pesticide. And you eat the plant that accumulated the pesticide. Hell, that may make you ill, but it's not the GM plant's fault, since it's the farmer who applies the pesticide!!)


    I have identified a lot of natural mechanisms over the last years from other organisms that we can put into the bee.
    3 Months and we have our first proof-of concept bee, totally resistant against some viruses and perhaps Nosema resistant.


    If you are interested, shoot me an email

    All the best,
    beemann

  2. #2
    Join Date
    Mar 2014
    Location
    Lembach, Austria
    Posts
    13

    Default Re: Job transgenic bee

    Btw, the end goal is a bee that is totally resistant to CCD.

    For what I read, bees have a very very small gene pool (caused by thier way of *more-or-less* in-breeding) compared to other inscects. They can not change their genomes as quick as other insects by natural breeding therefore they hardly have resistance mechanisms.

    I would love to reveal all the genetic circuits I have designed here, to make them open source. But problem is, then it would no longer be patentable. And I gotta make some money to be able to afford further modifying the bees.


    _________
    Just another anti-GMO disclaimer... An apple has around 57'000 genes - more or less depending on the species (I didn't count the genes from bees, but they're there http://www.ncbi.nlm.nih.gov/genome?t...is%20mellifera , one of the genes being this http://www.ncbi.nlm.nih.gov/nuccore/NM_001011578.1. If you add one gene, it is still is the same apple with just one additional protein. If you do natural breeding, half of the genes of the mother and half of the father are randomly mixed, genetic recombination occurs... Parasitc genetic elements (transposons) jump within the DNA into a new site ("jumping genes")... The result being actually much less predictable! If you're honest - chances are much much much higher that the naturally bred apple will be toxic than a GMO derivate from a known non-toxic apple
    Last edited by beemann; 03-27-2014 at 05:32 AM.

  3. #3
    Join Date
    Dec 2005
    Location
    Brasher Falls, NY, USA
    Posts
    28,388

    Default Re: Job transgenic bee

    I predict that it would be easier to reach your goal and ignore CCD. Concentrate on those things we know about and can identify, like nosema, varroa, and viruses. If beekeepers successfully control those things which they can have some modicum of control over then their bees aught to survive well.

    Best wishes. If you are for real.
    Mark Berninghausen
    The answers are the end. The questions are the journey. Journey on.



  4. #4
    Join Date
    Mar 2014
    Location
    Lembach, Austria
    Posts
    13

    Default Re: Job transgenic bee

    Ok,
    If I wipe out many diseases, there may be less CCD? CCD has many factors... viruses will be the easist to wipe out. Then come bacteria, then nosema. Varroa will be the most difficult, but I already have four experimental plans on varroa (not guaranteed that any of the anti-varroa strategies will work). But aren't there some strains that fight off varroa to some degree? Hygenie strains IIRC.

    Thanks a lot! Actually if I succeed doesn't depend so much on me, rather if I can find a beekeeper willing to do this.... Most are natural enthusiasts (just like me, that's awesome) but also anti-GMO (not-so-good)

  5. #5
    Join Date
    Jul 2010
    Location
    Auckland,Auckland,New Zealand
    Posts
    6,173

    Default Re: Job transgenic bee

    If someone does the inseminations in another country, how are you going to run this from Austria?

    How is the GMO to be done? It would be a physical impossibility to do all the sperm cells then impregnate a queen, a better approach could be to manually fertilize an egg laid in a drone cell immediately after laying.

    How are you considering to do this?
    44 years, been commercial, outfits up to 4000 hives, now 120 hives and 200 nucs as a hobby, selling bees. T (mostly).

  6. #6
    Join Date
    Mar 2014
    Location
    Lembach, Austria
    Posts
    13

    Default Re: Job transgenic bee

    As I mentioned, for the job I and the beekeeper would need to go to Ireland. There are investors that would fund the party.

    After being laid, the cells are already multicellular.

  7. #7
    Join Date
    Mar 2014
    Location
    Lembach, Austria
    Posts
    13

    Default Re: Job transgenic bee

    Btw, you don't inject DNA into the sperm cells manually. That would be too much work. You add millions of sperm cells and million DNA fragments, and by special means the sperm takes up DNA. Google for heat shock transformation, electroporation, PEG transfection, Gene gun, etc. (I can't publicly tell which method I'll be using.) But getting the DNA into the cells is the easiest part.

    Then you inseminate the queen with the modified sperm cells.

  8. #8
    Join Date
    Oct 2013
    Location
    Raleigh, North Carolina, USA
    Posts
    186

    Default Re: Job transgenic bee

    This smells fishier than a can of sardines on a hot summer day.
    I eat my peas with honey. I’ve done it all my life. It makes the peas taste funny. But it keeps them on the knife!

  9. #9
    Join Date
    Apr 2010
    Location
    Warrior, Alabama
    Posts
    1,073

    Default Re: Job transgenic bee

    As a sidenote for the anti-gmo people: It is totally harmless for both the environment and for the bees. No bee is hurt! We are working tightly together with EPA and will never release a bee until we get approval to do so.
    (Inserted DNA is not dangerous in any way - it's just e.g. if a pesticide resistance is inserted into a plant, the farmer then uses the pesticide. And you eat the plant that accumulated the pesticide. Hell, that may make you ill, but it's not the GM plant's fault, since it's the farmer who applies the pesticide!!)


    Experience has taught that one of the most deadly agents to life is a room full of brilliant people.
    Old Guy in Alabama

  10. #10
    Join Date
    Jan 2005
    Location
    Hamilton, Alabama
    Posts
    1,233

    Default Re: Job transgenic bee

    I actually checked the date on my computer to see if it was April 1st.
    DarJones - 45 years, 10 colonies (max 40), sideliner, treatment free since 2005, 11 frame broodnest, small cell

  11. #11
    Join Date
    Jul 2013
    Location
    Mirabel, Québec, Canada
    Posts
    456

    Default Re: Job transgenic bee

    "Never release a bee until the EPA authorizes it"...

    First of all, you would never be conducting this research if people did not have the intent to ultimately release it. And governmental approval is more of a matter of "when" than a matter of "if".

    Second of all, I fail to see how you plan on containing these genetics.
    www.apisrustica.com (French-only website) Bee Breeding: Canadian nuclei & queens
    www.facebook.com/Apis.rustica

  12. #12
    Join Date
    May 2012
    Location
    Sacramento, CA, USA
    Posts
    3,063

    Default Re: Job transgenic bee

    It only costs about 35 million to deregulate an "event" here and even then you have to get approval from countries to import, and on top of that you can't even import bees here anyways..... The best of luck but transforming many traits into an organism in a stable fashion and getting a good end product isn't as simple as you're making it out.

  13. #13
    Join Date
    Mar 2014
    Location
    Lembach, Austria
    Posts
    13

    Default Re: Job transgenic bee

    The agreement is they own 20% of the company then. That means I have more than 50% which means my decision will be followed. I assume, but I haven't studied MBA

  14. #14
    Join Date
    May 2012
    Location
    Sacramento, CA, USA
    Posts
    3,063

    Default Re: Job transgenic bee

    Stewardship will be your biggest problem, trust me. When your bees 'genetics' show up in unapproved countries, guess who gets the bill. I'm also telling you, the attitude towards GMO for most people is, 'they don't want it'. If you think getting a GMO product to market is as straight forward as you're making it sound you're fooling yourself. Just start talking to all the regulatory agencies involved to get a product out, and your head will spin in the first 30 seconds.

  15. #15
    Join Date
    Mar 2014
    Location
    Lembach, Austria
    Posts
    13

    Default Re: Job transgenic bee

    You don't think it's worth trying? Or fight for your dreams? Some country will allow it.

  16. #16
    Join Date
    Dec 2012
    Location
    Sacramento, CA, USA
    Posts
    2,093

    Default Re: Job transgenic bee

    In a season I can select up to 4 generation of bees to play with simultaneously. That is my ongoing dream although not a
    big one now. Since it is of natural selection I don't have to worry much about their genetic manipulation. All the good characteristics are here after 40 years of natural selection. All I need to do is to continue to select from the best one to continue this progress.
    Everything is contained locally for my own learning and research only. Of course there are many countries that will allow the
    people who have their dreams and the knowledge to try it. While you are still dreaming many people are doing it the natural
    way with AI, II already improving their stocks. Before you can improve you need to know what you are looking for first. How many
    years have you had hands on experience with the honey bees? I don't think you will allow your assistant beekeepers to handle such an important work. Do you?
    I luv bee source!

  17. #17
    Join Date
    Mar 2014
    Location
    Lembach, Austria
    Posts
    13

    Default Re: Job transgenic bee

    Quote Originally Posted by beepro View Post
    In a season I can select up to 4 generation of bees to play with simultaneously. That is my ongoing dream although not a
    big one now. Since it is of natural selection I don't have to worry much about their genetic manipulation. All the good characteristics are here after 40 years of natural selection. All I need to do is to continue to select from the best one to continue this progress.
    Everything is contained locally for my own learning and research only. Of course there are many countries that will allow the
    people who have their dreams and the knowledge to try it. While you are still dreaming many people are doing it the natural
    way with AI, II already improving their stocks. Before you can improve you need to know what you are looking for first. How many
    years have you had hands on experience with the honey bees? I don't think you will allow your assistant beekeepers to handle such an important work. Do you?
    since it is of natural selection I don't have to worry much about their genetic manipulation.
    This is a common fallacy. When doing natural interbreeding, half of the genome (mother) recombines with half of the other genome (dad). There are weird things going on, namely jumping genes, retrotransposons, non-homologous end joining (aka illegimate recombination - DNA breaks and gets repaired, sometimes with errors), polymerase errors (mutation)...

    http://en.wikipedia.org/wiki/Naturalistic_fallacy

    While you are still dreaming many people are doing it the natural
    way with AI, II already improving their stocks.
    And this is here the problem is. I don't believe you can breed new traits fast enough. Else there would be no CCD any more already.


    EDIT: Deleted confidental information. Sry.
    Last edited by beemann; 04-10-2014 at 07:18 AM.

Bookmarks

Posting Permissions

  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts
  •  
Ads