Results 1 to 5 of 5
  1. #1
    Join Date
    Jan 2010
    Location
    Alachua County, FL, USA
    Posts
    7,131

    Default A Comparison of Digital Gene Expression Profiling and Methyl DNA Immunoprecipitation

    A Comparison of Digital Gene Expression Profiling and Methyl DNA Immunoprecipitation as Methods for Gene Discovery in Honeybee (Apis mellifera) Behavioral Genomic Analyses
    The honey bee has a well-organized system of division of labor among workers. Workers typically progress through a series of discrete behavioral castes as they age, and this has become an important case study for exploring how dynamic changes in gene expression can influence behavior. Here we applied both digital gene expression analysis and methyl DNA immunoprecipitation analysis to nurse, forager and reverted nurse bees (nurses that have returned to the nursing state after a period spent foraging) from the same colony in order to compare the outcomes of these different forms of genomic analysis. A total of 874 and 710 significantly differentially expressed genes were identified in forager/nurse and reverted nurse/forager comparisons respectively. Of these, 229 genes exhibited reversed directions of gene expression differences between the forager/nurse and reverted nurse/forager comparisons. Using methyl-DNA immunoprecipitation combined with high-throughput sequencing (MeDIP-seq) we identified 366 and 442 significantly differentially methylated genes in forager/nurse and reverted nurse/forager comparisons respectively. Of these, 165 genes were identified as differentially methylated in both comparisons. However, very few genes were identified as both differentially expressed and differentially methylated in our comparisons of nurses and foragers. These findings confirm that changes in both gene expression and DNA methylation are involved in the nurse and forager behavioral castes, but the different analytical methods reveal quite distinct sets of candidate genes.
    http://www.plosone.org/article/info%...l.pone.0073628
    americasbeekeeper.com
    beekeeper@americasbeekeeper.com

  2. #2
    Join Date
    Dec 2012
    Location
    Fort Walton Beach, Florida
    Posts
    1,256

    Default Re: A Comparison of Digital Gene Expression Profiling and Methyl DNA Immunoprecipitat

    I really appreciate these posts... thank you. Very interesting stuff!

  3. #3
    Join Date
    Sep 2008
    Location
    Mtn. View, Arkansas, USA
    Posts
    1,319

    Default Re: A Comparison of Digital Gene Expression Profiling and Methyl DNA Immunoprecipitat

    Hay AmericasBeekeeper; Are you allowed to do a synopsis and translate your posts in to Redneck English for those among us that are mentally challenged?

  4. #4
    Join Date
    Aug 2002
    Location
    Nehawka, Nebraska USA
    Posts
    46,785

    Default Re: A Comparison of Digital Gene Expression Profiling and Methyl DNA Immunoprecipitat

    > Are you allowed to do a synopsis and translate your posts in to Redneck English for those among us that are mentally challenged?

    That should be a choice on google translate... Redneck English, Spanglish, Ebonics...
    Michael Bush bushfarms.com/bees.htm "Everything works if you let it." ThePracticalBeekeeper.com 40y 200h 37yTF

  5. #5
    Join Date
    Feb 2010
    Location
    New York City, NY
    Posts
    4,317

    Default Re: A Comparison of Digital Gene Expression Profiling and Methyl DNA Immunoprecipitat

    They're trying to figure out which genes get turned on/off for the different sub-castes of Honeybee workers.

    (forager/nurse and reverted nurse/forager).

Tags for this Thread

Bookmarks

Posting Permissions

  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts
  •  
Ads