A help is welcome. The question is simple: I find a technique that can help me maintaining VSH traits.
The technique as you can see presupposes one mating flight (1). Some experts state that the queen under natural conditions only makes one flight (2) and (3), yet others report several mating flights (4).
(1) A novel system for the control of natural mating of A. mellifera queens has been developed by Mr Jo Horner, an Australian queen breeder in Rylstone, New South Wales. The system allows up to 240 queens to be control mated on a single day, which is far greater than can be achieved by instrumental insemination. Furthermore, the system does not require geographical isolation. Instead, Horner's system controls natural mating of queens and drones by manipulating the time that they undertake mating flights. Under natural mating, drones of A. mellifera start their mating flights shortly after noon and continue until 1630 or 1700 h (Koeniger et al. 2005). Males gather at drone congregation areas—specific areas in the landscape (Loper et al. 1987, Pechhacker 1994) that attract hundreds or thousands of unrelated drones (Baudry et al. 1998). Virgin queens fly to a congregation area where they mate before returning to their original colony. In http://jhered.oxfordjournals.org/content/101/3/334.full
(2) As far as known, Apis species mate at DCAs (review Koeniger and Koeniger, 2000). Male aggregations seem to facilitate and ensure a rapid mating of queens with many drones during one successful mating flight of 15 to 30 min (A. koschevnikovi: Koeniger et al., 1994a; A. dorsata: Tan et al., 1999; A. cerana: Woyke, 1975; Punchihewa et al., 1990; A. mellifera: Woyke, 1960). in http://www.apidologie.org/index.php option=com_article&access=standard&Itemid=129&url=/articles/apido/pdf/2005/02/M4070.pdf
(3) "Virgin queens mate early in their lives and only attend one mating flight. After several matings during this flight, a queen stores up to 100 million sperm within her oviducts. However, only five to six million are stored within the queen’s spermatheca. The queen uses only a few of these sperm at a time in order to fertilize eggs throughout her life." In http://www.orkin.com/stinging-pests/...ey-bee-mating/
(4) "A honey bee colony consists of a single queen and several thousand sterile worker bees. Throughout most of her life, the queen’s job is to lay eggs. However, early in a queen’s life, she makes several mating flights. On these flights, she mates — in midair — with anywhere from one to more than 40 drones. The average number of drones with which a queen mates is 12. The queen stores the semen from her mating flights for the remainder of her life, two-to-three years for a long-lived queen." In http://www.cals.ncsu.edu/agcomm/maga.../n_mating.html