Results 1 to 9 of 9
  1. #1
    Join Date
    Nov 2009
    Location
    Halifax, Nova Scotia, Canada
    Posts
    1,974

    Default Home VSH Testing

    I have been doing some reading on VSH testing, and it seems that getting the gear to do testing with liquid nitrogen is a bit rich for my neighborhood. Here's a pdf which describes tests

    So I'm considering the old freezer technique.

    Anyone do it?

    What are your thoughts on that test?

    Adam

  2. #2
    Join Date
    Jul 2013
    Location
    Morro Bay, California, USA
    Posts
    839

  3. #3
    Join Date
    Dec 2006
    Location
    St. Albans, Vermont
    Posts
    5,375

    Default Re: Home VSH Testing

    The hygienic response for brood removal, and VSH removal of mite infested brood are not the same.

  4. #4
    Join Date
    Sep 2007
    Location
    New Albany, Ohio, USA
    Posts
    351

    Default Re: Home VSH Testing

    Yeah, what Mike said.

    Hygienic behavior for controlling brood diseases involves the liquid nitrogen frozen brood test or some derivation of it.

    Selection for VSH behavior is going to set you back a little more money and time as a scope is very helpful and a good bit of time to do the dissection or brood and counting.

    Joe
    Breeder Queens & Honey Bee Nutritional Supplements
    www.latshawapiaries.com

  5. #5
    Join Date
    Nov 2012
    Location
    Springfield, Ohio, USA
    Posts
    458

    Default Re: Home VSH Testing

    liquid nitrogen is nice because it freezes quickly, then boils off quickly. If you're wanting to test for brood removal, perhaps dry ice would be an acceptable substitute. It can be pulverized into a granular powder easily by placing into a sturdy container, and whacking it with the top of a claw hammer or piece of wood. Shaking the container the way one would flip an egg in a skillet without a spatula will raise any un-pulverized chunks to the surface for another round of pulverization. Or, you could sift through 1/4" hardware cloth.

    If you do use the dry ice, know that because it takes longer to sublime than liquid nitrogen does to boil, the area that will be frozen will be a bit larger than the area of application.
    Pete. New 2013, 7 hives, zone 6a
    To study and not think is a waste. To think and not study is dangerous.

  6. #6
    Join Date
    Aug 2002
    Location
    Nehawka, Nebraska USA
    Posts
    46,120

    Default Re: Home VSH Testing

    > perhaps dry ice would be an acceptable substitute.

    When discussing this with Marla Spivak she said dry ice did not work well. It often did not kill the brood. If you really don't want to buy the dewar and the liquid nitrogen, I'd either do the pin prick method (which Marla says isn't as good because there are two behaviors involved and one is uncapping which you skip with this method) or cut the section out and freeze it in your freezer overnight, thaw it, and then put it back in the hole you made and see how fast they uncap it.
    Michael Bush bushfarms.com/bees.htm "Everything works if you let it." ThePracticalBeekeeper.com 40y 200h 37yTF

  7. #7
    Join Date
    Sep 2005
    Location
    Greensboro, North Carolina
    Posts
    2,723

    Default Re: Home VSH Testing

    Marla Spivak recommended against using dry ice at last year's EAS in Vermont. Instead, she suggested using a round cookie cutter to punch out a section of capped brood from a frame (of wax foundation without wires), put it in a freezer overnight, then put it back in place the next day (after thawing).

    She actually recomended using the cookie cutter over liquid nitrogen for people that weren't doing dozens of tests a day. She said that she had to use the nitrogen because she couldn't wait 24 hours and then revisit the same site.

  8. #8
    Join Date
    Sep 2005
    Location
    Greensboro, North Carolina
    Posts
    2,723

    Default Re: Home VSH Testing

    You beat me to it Mike, lol.

  9. #9
    Join Date
    Nov 2009
    Location
    Halifax, Nova Scotia, Canada
    Posts
    1,974

    Default Re: Home VSH Testing

    Mike Palmer and Joe Latshaw - yes. You are correct and that was my error in typing the original post. I should have said "hygenic testing" and not "VSH testing".

    True. Hygenic and Varroa Sensitive Hygiene are not the same.

    Adam

Bookmarks

Posting Permissions

  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts
  •  
Ads