You now have queens piping. That generally indicates that the old queen already left with a swarm. A new virgin is in the hive and there are multiple queens confined in cells that are still alive. If you disturb the hive, those queens that are confined will likely escape. The outcome of that is difficult to predict. If this was several days ago and they have not swarmed and the weather is nice, that virgin queen has likely killed the others and they are likely now without a laying queen until she mates. They are also likely without the means to raise another queen (no eggs or young larvae). I would be very careful doing a split right now and would make sure there are eggs and open brood in both halves of the split (even if you have to take them from somewhere else).

The most important aspect of a cut down split is timing. I should be done two weeks before the flow, ideally, and no later than right after the flow starts. My guess is that time is past...

http://bushfarms.com/beessplits.htm#cutdown